Microfluidic Devices Made of Uv-curable Glue (noa81) for Fluorescence Detection Based Applications

نویسندگان

  • Ph. Wägli
  • B. Y. Guélat
  • A. Homsy
  • N. F. de Rooij
چکیده

The UV-curable adhesive NOA81 (Norland Optical Adhesive) is a promising liquid photopolymer for low-cost microfluidic chip production. Different fabrication methods for NOA81 are suggested [1,2]. However, optical characterization for fluorescent based detection systems is missing. We present a comparison of fluorescent emission spectra of various NOA types as well as the time evolution of the spectra of the most promising one NOA81. We report on a simple rapid prototyping technique to produce chips for fluorescent based capillary electrophoresis (CE). As an application, we show the CE-injection of a fluorescent dye, and its detection by fluorescence microscopy.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Soft lithography fabrication of index‐matched microfluidic devices for reducing artifacts in fluorescence and quantitative phase imaging Diane N. H. Kim1 · Kevin T. Kim2 · Carolyn Kim1 · Michael A. Teitell1,3 · Thomas A. Zangle4

Microfluidic devices are widely used for biomedical applications based on microscopy or other optical detection methods. However, the materials commonly used for microfabrication typically have a high refractive index relative to water, which can create artifacts at device edges and limit applicability to applications requiring high-precision imaging or morphological feature detection. Here we ...

متن کامل

Low Temperature “click” Wafer Bonding of Off-stoichiometry Thiol-ene (oste) Polymers to Silicon

INTRODUCTION Common bonding techniques for lab-on-chip (LOC) microfluidic devices require surface bio-functionalization to be performed in-situ after the chip has been packaged due to the bio-incompatible features of the bonding technique, including high temperature requirements (e.g. thermal bonding of thermoplastics), use of organic solvents (e.g. PMMA bonding) or plasma activation (e.g. PDMS...

متن کامل

Deep UV laser-induced fluorescence detection of unlabeled drugs and proteins in microchip electrophoresis.

Deep UV fluorescence detection at 266-nm excitation wavelength has been realized for sensitive detection in microchip electrophoresis. For this purpose, an epifluorescence setup was developed enabling the coupling of a deep UV laser into a commercial fluorescence microscope. Deep UV laser excitation utilizing a frequency quadrupled pulsed laser operating at 266 nm shows an impressive performanc...

متن کامل

Inkjet printing of UV-curable adhesive and dielectric inks for microfluidic devices.

Bonding of polymer-based microfluidics to polymer substrates still poses a challenge for Lab-On-a-Chip applications. Especially, when sensing elements are incorporated, patterned deposition of adhesives with curing at ambient conditions is required. Here, we demonstrate a fabrication method for fully printed microfluidic systems with sensing elements using inkjet and stereolithographic 3D-print...

متن کامل

A PDMS-Based Cylindrical Hybrid Lens for Enhanced Fluorescence Detection in Microfluidic Systems

Microfluidic systems based on fluorescence detection have been developed and applied for many biological and chemical applications. Because of the tiny amount of sample in the system; the induced fluorescence can be weak. Therefore, most microfluidic systems deploy multiple optical components or sophisticated equipment to enhance the efficiency of fluorescence detection. However, these strategi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2010